Atamt1 1

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August undefined, 2024

WebDec 5, 2006 · Preferential Localization of AtAMT1;2 in the Plasma Membrane of Endodermal Root Cells. The transcripts of several NH 4 + transporters have been identified in roots, including those of AtAMT1;1 and AtAMT1;2 (Gazzarrini et al., 1999).In apical zones of the roots, the green fluorescent protein (GFP) fusion protein with AtAMT1;1 under the … WebThe NH 4 1 concentration needed to saturate AtAMT1;2 was much higher than for AtAMT1;1, which has a more than 10-fold higher affinity (Mayer and Ludewig, 2006; …

Nitrogen-Dependent Posttranscriptional Regulation of …

WebDec 15, 2006 · The high AtAMT1;1 transcript levels in the two transgenic lines 18 and 19 led to accumulation of large amounts of AtAMT1;1 protein, as determined by protein gel-blot analysis using a rabbit antibody targeted against 15 amino acid residues from the loop between the transmembrane-spanning helices 2 and 3 of AtAMT1;1 . This serum was … WebNov 1, 2002 · Results are the first in plant evidence that AtAMT1;1 is a root NH4 + transporter and that redundancies within the AMT family may allow compensation for the loss of AtAMMT 1;1. NH4 + acquisition by plant roots is thought to involve members of the NH4 +transporter family (AMT) found in plants, yeast, bacteria, and mammals. In … fielding cast iron

Temporal clustering by affinity propagation reveals transcriptional ...

WebEngineer C B, Kranz R G (2007) Reciprocal leaf and root expression of AtAmt1.1 and root architectural changes in response to nitrogen starvation. Plant Physiology 143:236–250 Gifford M L, Dean A, Gutierrez R A, Coruzzi G M, Birnbaum K D (2008) Cell-specific nitrogen responses mediate developmental plasticity. Proc. Natl. Acad. WebJan 1, 2024 · that AtAMT1;1 is a high-aﬃnity ammonium transporter (Wood e t al. 2006). Disruption of AtAMT1;1 was found to . result in ~ 70% ammonium absor ption capacity retention in . WebJan 1, 2006 · AtAMT1;1 and AtAMT1;3 are located in the plasma membrane of the epidermal and cortical cell layer of the root (Mayer and Ludewig 2006; Loqué et al. 2006). AtAMT1;2 localization is restricted to ... grey round kitchen table

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High-affinity ammonium transport by Arabidopsis …

WebJan 20, 2014 · AtAMT2-1 has ammonium transport activity similar to that of AtAMT1-1 at pH 7.5, but transport capacity of AtAMT2-1 is an order of magnitude lower than that of AtAMT1-1 at pH 6.5 . The monocot group A-I appears to have undergone an early duplication event preceding speciation in the monocots, leading to two sister clades (Figure 4A). WebDec 4, 2024 · AtAMT1;1 was up-regulated under nitrogen deficiency (Shelden et al. 2001) and mediated ammonium influx (km = 34 µM), indicating that AtAMT1;1 is a high-affinity ammonium transporter (Wood et al. 2006). Disruption of AtAMT1;1 was found to result in ~ 70% ammonium absorption capacity retention in Arabidopis roots (Kaiser 2002). grey round kitchen table setWebSep 5, 2024 · Similar results were also found with AtAMT1;1, AtAMT1;2 and AtAMT1;3 (Loqué et al. 2007; Neuhäuser et al. 2007; Yuan et al. 2013). However, the transportation activity is still unknown after being phosphorylated of S449 of AtAMT1.1 in Arabidopsis, to our knowledge so far. How about the phosphorylation effect of homologous site S458 of … fielding chemicals canada

"WebNov 3, 2006 · AtAmt1.1 functions as a saturable high-affinity transporter with a K m of 65 μ m for methylamine, an ammonium analog. Using AtAmt1.1, two homologous cDNAs, … " - Atamt1 1

Atamt1 1

High-affinity ammonium transport by Arabidopsis …

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WebNov 3, 2006 · Abstract. Nitrogen is an essential macronutrient for plant growth and survival. Here, the temporal and spatial sensing of nitrogen starvation is analyzed in Arabidopsis (Arabidopsis thaliana).The promoter for the high-affinity ammonium transporter, AtAmt1.1, is shown to be a valid indicator for nitrogen status in leaves and roots.An AtAmt1.1-Gal4 … WebAnnotation: AMT1;1 locus:2140877 AT4G13510 AT4G13510 AMT1;1 ATAMT1 ATAMT1;1 ammonium transporter 1;1 ARABIDOPSIS THALIANA AMMONIUM TRANSPORT 1 T6G15.60 T6G15_60

WebApr 1, 2001 · Unlike AtNrt2.1, AtAmt1.1 expression is not stimulated in a nitrate reductase-deficient mutant after transfer to NO3- as sole N source, indicating that NO3- per se acts as a signal repressing transcription of AtAmt1.1. These results reveal two fundamentally different types of mechanism involved in the feedback regulation of root N acquisition ... WebJan 25, 2024 · CPK32 could interact with AtAMT1.1 and phosphorylate AtAMT1.1 at the non-conserved serine residue Ser450 in the CTR to enhance ammonium uptake in the natural environment (Qin et al. 2024). This study revealed a new mechanism underlying regulation of normal ammonium uptake. Taken together, the posttranslational regulation …

WebThe NH 4 1 concentration needed to saturate AtAMT1;2 was much higher than for AtAMT1;1, which has a more than 10-fold higher affinity (Mayer and Ludewig, 2006; Wood et al., 2006). WebDec 5, 2006 · AtAMT1;2 functions as a comparatively low-affinity NH4+transporter. Mutations at the highly conserved carboxyl terminus (C terminus) of AMTs, including one …

WebJan 4, 2024 · Switching to the expression of AtAMT1;1 driven by ProAtUBQ10 or of AtAMT1;1 mutated at two siRNA‐targeted hotspots reduced AtAMT1;1‐specific siRNAs and overcame S‐PTGS in roots. In roots of these lines, however, the steady‐state transcript levels of AtAMT1;1 still significantly decreased under conditions of N‐sufficiency …

WebDifferential regulation of AtNrt2.1 and AtAmt1.1 is found in the same plant At least two hypotheses can be raised to explain the differential regulation of AtNrt2.1 and AtAmt1.1 … grey round landscaping rockWebNational Center for Biotechnology Information grey round mirrorWebSep 1, 2024 · While AtAMT1–1 and 1–3 exhibited more similarity than AtAMT1–4, AtAMT1–4 was separated from all AMT1s in group B. In view of exon numbers, it was seen that the S1AMT1–2 gene contains only two exons, and this difference was observed in the phylogenetic tree, proposing that gene variations may cause this differentiation. grey round mirrors ukWebDec 10, 2008 · Total RNA (20–30 μ g per lane) was separated by formaldehyde agarose gel electrophoresis, and RNA was transferred to a nylon membrane. DNA fragments carrying the ORFs of AtAMT1;1 or AtAMT1;4 were labeled with 32 P as probes for RNA hybridization. Preparation of membrane fractions and protein gel blot analysis. Total … fielding chartWebthe soil to the vasculature 1,2. AtAMT1;1 and AtAMT1;3 are located in the plasma membrane of the epidermal and cortical cell layer of the root 3,4. AtAMT1;2 localization is restricted to the cortical and endodermal root cells 1. The localization and high affinity of AtAMT1;1 (In oocytes: K m = 2.7 µM 3; in plants: K m = 50.0 µM fielding cemetery searchWebJul 1, 2006 · AtAMT1;1 was the founding member of the family of AMT/Rh ammonium transporters and accounts for about one third of the total ammonium absorption in the roots of the model plant Arabidopsis. Recent evidence suggested that at least some AMT/Rh proteins are NH3 gas channels. In order to evaluate the transported form of ammonium … fielding chemical technologies inc careersWebMay 21, 2024 · Figure 1. NH 4 + currents recorded with different recording strategies in oocytes. AtAMT1;3-expressing oocytes were subjected to 2 successive cycles of 2 perfusion solutions, the first solution being free from NH 4 + and the second one containing 1 mM NH 4 + (same solution background, pH 7.4). For each cycle, the oocyte was successively … fielding chemicals