WebThe antibody mixture reacts with human IgA (α-chains), IgG (γ-chains), IgM (μ-chains), and with kappa and lambda light chains. Traces of contaminating antibodies have been removed by solid-phase absorption. In crossed immunoelectrophoresis, the antibody specificity of each of the five individual FITC-conjugates was ascertained, prior to FITC ... WebThe HPLC chromatograms of 10 À4 M FITC and probe. Chromatographic conditions: the mobile phase consists of acetonitrile and ultrapure water (v:v = 6:4, 1% acetic acid). The flow rate was 1 mL min ...

FITC labeling of human insulin and transport of FITC

WebJun 15, 2008 · In a HPLC glass vial, 300 μl of reconstituted residue was derivatised with 300 μl of FITC (20 mM). The mixture was vortex for 40 s, and incubated at 80 °C in the oven … WebFITC-dextran has been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran. Fluorescein isothiocyanate–dextran (FITC-Dextran 4) is use as a fluorescent probe to study cell permeability. FITC-Dextran 4 is used with to study permeability of epithelial cell such as ... how do lisps occur

Mass Spectrometry-Based Solid Phase Peptide Reaction Assay for …

WebThe Thermo Scientific FITC Antibody Labeling Kit is a multi-component kit containing a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of … WebJan 21, 2009 · Abstract. Fluorescein isothiocyanate (FITC) is an amine reactive derivative of fluorescein dye that has wide ranging application in biochemistry. It has been extensively used to label peptides and proteins. However, its use in solid phase peptide synthesis is restricted. Indeed, in acidic conditions required for linker cleavage, N-ter FITC ... WebJun 15, 2008 · In a HPLC glass vial, 300 μl of reconstituted residue was derivatised with 300 μl of FITC (20 mM). The mixture was vortex for 40 s, and incubated at 80 °C in the oven for 20 min. After cooling, 100 μl of the derivatised sample was injected into the HPLC system and separated at ambient temperature, using a constant flow rate of 1 ml/min. how much potassium is in tea