Phospho irf3
WebPhospho-IRF3 (Ser396) Antibody (BS-3195R) in WB Mouse liver lysates probed with Anti-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:300 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37°C. Product Details Target Information WebMar 30, 2024 · Similarly, the IRF7, IRF1, and phospho-IRF3 levels hiked after two days of SARS-CoV-2 infection. These findings suggested that IRF7, IRF1, and IRF3 were stimulated following HCoV infections.
Phospho irf3
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WebIRF-3 is a member of the interferon regulatory transcription factor (IRF) family. It mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity. DsRNA generated during the course of a viral infection leads to IRF-3 phosphorylation on the C-terminal serine/threonine cluster. WebSep 19, 2013 · IRF3 is a constitutively expressed transcription factor that resides in the cytoplasm and dimerizes and translocates to the nucleus upon phosphorylation ( 6 ). …
WebWestern Blot using Phospho-IRF3 (Ser386) Polyclonal Antibody ( Product # PA5-78025 ). Untreated (–) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Phospho-IRF3 (Ser386) Polyclonal Antibody ( Product # PA5-78025) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody ... WebApr 28, 2024 · Immunoblotting experiments indicated that phosphorylation of IRF3 S386, which is a hallmark of cGAS-mediated activation of downstream events 21, ... (Histone H3 phospho S10) and cyclin B was used ...
WebSep 1, 2024 · To systematically search for the phosphorylation sites on IRF3 in vivo, we carried out tandem affinity purification of recombinant double-tagged IRF3 expressed in HEK293 cells, coupling with mass spectrometric analysis (Fig. 1 A and B). Several phospho-peptides were identified with different color highlighted in the whole amino acid sequence … WebIntracellular Delivery of Lipopolysaccharide Induces Effective Th1-Immune Responses Independent of IL-12 Sachiko Watanabe1., Joe Inoue1,2*. 1Department of Biosciences, School of Science and Graduate School of Science, Kitasato University, Japan, 2Department of Immunology and Cell Biology, Graduate
WebThe phospho-IRF3 cell based assay kit conveniently and accurately quantifies phosphorylated IRF3 at Ser386. In viral infected cells, phosphorylation of transcription …
WebPhosphorylated IRF3 forms a dimer that translocates to the nucleus and induces type I IFNs and other cytokines. After activation by STING, TBK1 and its homolog IκB kinase epsilon (IKKε) can both lead to activation of the IKK complex, which then activates the transcription factor nuclear factor κB (NF-κB) (11, 12). dunwhinny gardens peeblesWebApr 23, 2024 · We focused on the phosphorylation of IRF3, which is a downstream factor of the cGAS pathway . As shown in Figure 2A, the expression of phosphorylated-IRF3 (p-IRF3) was increased in HNSCC cells after X-ray irradiation, and cGAS knockdown suppressed the radiation-increased p-IRF3. These results suggest that ionizing radiation activates IRF3 ... dunwich amps volt throwerWeb55. A method of treating, inhibiting, reducing, ameliorating, and/or preventing a cancer and or metastasis in a subject comprising administering to a subject an adoptive immunothe dunwich accommodationWebApr 13, 2024 · Antibodies. The following antibodies were used: mouse monoclonal antibodies to α-tubulin (Santa Cruz, sc23948), GAPDH (Santa Cruz, sc32233), phospho-H2A.X (Ser139) (EMD Millipore, 05-636), anti ... dunwich asylum inmates recordsWebPhospho-IRF3 (S386) ELISA Kit (ab279833) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human … dunwhich horror settingWebWestern blot analysis of extracts of NIH/3T3 cells, using Phospho-IRF3-S396 antibody (AP0623) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. dunwich arkham horrorWebIRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. … dunwich bakery stradbroke island