Phospho-stat3 tyr705
WebSTAT3 is activated by Tyr705 phosphorylation in response to membrane factors such as interleukin-6 (IL-6). 45 It has also been shown that Ser727 of STAT3 is phosphorylated by various kinds of kinases in various cancers. 46–49 However, some reports have shown that the Ser727 site phosphorylation suppresses STAT3 activity and cell proliferation ... WebAlexa Fluor® 488 anti-STAT3 Phospho (Tyr705) Antibody. Human whole blood was stimulated with (top) or without (bottom) IL-6 for 15 minutes, then treated with RBC Lysis/Fixation Solution and permeabilized with True-Phos™ Perm Buffer, then stained with CD3 APC and STAT3 pY705 (clone 13A3-1) Alexa Fluor® 488. Compare all formats See …
Phospho-stat3 tyr705
Did you know?
WebDec 1, 2024 · A model for STAT3 phosphorylation at Ser727 and Tyr705 differentially regulating the EMT–MET switch and cancer metastasis. Model proposing a pathway in which heterogeneous CD151 + /CD38 + and CD133 + /CD83 + CSC subtypes undergo STAT3/pY705-elicited EMT and STAT3/pS727-elicited MET, respectively, to achieve … WebTest was conducted on a previous lot. Use Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB to detect phospho-STAT3 …
WebApr 12, 2024 · The effect of FLU on STAT3 expression and activation in A172 and T98G cells. (A) The time dependence of FLU effect on STAT3 and phospho-STAT3 (Tyr705) … WebPhospho-STAT3 (Tyr705) Kit. For Research Use Only. Not for use in diagnostic procedures. A randomized, placebo-controlled study of the pharmacokinetics, pharmacodynamics, …
WebApr 12, 2024 · The effect of FLU on STAT3 expression and activation in A172 and T98G cells. (A) The time dependence of FLU effect on STAT3 and phospho-STAT3 (Tyr705) expression. Cell were treated with 0.5 µM ... WebApr 23, 2012 · In the STAT3 (Tyr705) In-Cell ELISA Kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, Anti-Phospho-STAT3 (Tyr705) or Anti-STAT3 is pipetted into the wells and incubated. The wells are washed, and HRP-conjugated anti-mouse IgG is added to the wells.
WebJun 2, 2024 · Mechanistically, phosphorylated STAT3 (Tyr705) inhibition resulting from pimozide treatment caused a downregulation of downstream transcriptional targets such as matrix metalloproteinase-9 (MMP-9) and vimentin, both implicated in …
WebOct 14, 2024 · Phospho-Tyr705 (pY705) stabilizes the STAT3 dimer with reciprocal interactions between pY705 and the SH2 of the other molecule and phospho-Ser727 (pS727) accelerates pY705 dephosphorylation. We study how pS727 regulates STAT3 in both structural and biological perspectives. Using STAT3 reconstituted in HepG2-stat3 … portable scene lightingWebSTAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer … portable schoolWebJun 2, 2024 · Mechanistically, phosphorylated STAT3 (Tyr705) inhibition resulting from pimozide treatment caused a downregulation of downstream transcriptional targets such … portable school ovenWebSep 28, 2016 · Phospho-STAT3 (Tyr705), STAT3, GRP78 and GAPDH levels were analyzed by Western blotting. (A) Leptin-induced STAT3 phosphorylation in insulin-treated cells was ameliorated in GRP78-knocked down cells. portable school crosswalk signWebThe transcription factor signal transducer and activator of transcription 3 (STAT3) has two important phosphorylation sites, Tyr705 and Ser727, for its activation. Ser727 phosphorylation has been considered to be a secondary event after Tyr705 phosphorylation. In this study, the role and regulation … portable scanning wandWebWestern blot analysis of extracts from IFN-alpha treated Jurkat cells and HeLa cells (left), as well as EGF treated A431 cells (right), using Phospho-Stat3 (Tyr705) (D3A7) XP ® Rabbit mAb. Note that the basal phospho-Stat3 in A431 is detected by the antibody. Data were generated using the standard formulation of this product. Show Less Show More irs check stimulus 2022WebThe STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation of human STAT3 protein and has been shown to be useful for Western blotting. During PD testing, this clone failed to stain PFA-fixed cells that had been permeabilized with Triton X-100. We highly recommend permeabilizing cells with methanol. Product Citations irs check stimulus 2021