Pip2a-mcherry
Webb13 apr. 2024 · (A) Confocal microscopy of epidermis of Nicotianna benthamiana transiently expressing 35S:NILR1-GFP and plasma membrane marker 35S:PIP2A-mCherry. Scale, … WebbAAV is usually considered BSL-1, but may require BSL-259 handling depending on the insert. Storage. Store at -80°C. Thaw just before use and keep on ice. Quality Control. …
Pip2a-mcherry
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Webb28 juli 2024 · PIP2A–mCherry was used as a plasma membrane marker. ZmYSL2–enhanced green fluorescent protien (eGFP) and PIP2A–mCherry were excited … Webb2 juni 2024 · GFP and mCherry emission signals were detected with HyD (hybrid) detectors ranging from 500 to 530 nm and 580 to 650 nm, respectively. In planta overexpression of MjMCM2
Webb10 aug. 2024 · We found that sfGFP, Venus, mCherry and mCherry2 all caused substantial foci formation, despite being monomers or very weak dimers when expressed alone. mKate2 and TagRFP-T caused … Webb28 jan. 2024 · (a) Co-localization of P3a and PIP2A-mCherry. PIP2A acts as a PM marker; ( b ) Co-localization of P3a and its interacting proteins. AtPUP14-CFP, AtGTR1-CFP, and AtNRT1.7-CFP were co-expressed with P3a-RFP in N. benthamiana leaves; ( c ) Subcellular localizations of AtPUP14-CFP, AtGTR1-CFP, and AtNRT1.7-CFP during BrYV infection; …
Webb3 feb. 2024 · Constitutively expressed mCherry (proUBQ10::PIP2A-mCherry) was the screenable marker used to identify transformed seeds. Sequences of the T-DNAs … Webb4 nov. 2024 · a member of the plasma membrane intrinsic protein subfamily PIP2. localizes to the plasma membrane and exhibits water transport activity in Xenopus oocyte. …
Webb24 mars 2024 · Due to the lack of fluorescent marker‐tagged lines of cotton and the large diameter of cotton roots, we observed the initial infection process (16–24 h …
Webb12 juni 2013 · Copper (Cu) homeostasis in plants is maintained by at least two mechanisms: (1) the miRNA-dependent reallocation of intracellular Cu among major Cu-enzymes and important energy-related functions; (2) the regulation of the expression of Cu transporters including members of the CTR/COPT family. These events are controlled by … brass better than stainless steelWebb17 mars 2016 · Roots or leaves of 2-week-old A. thaliana plants expressing PIP2A-mCherry were inoculated with Ct-GFP spores (2–5 × 10 5 /ml) and imaged by confocal … brass bic lighter coverWebbPIP2;1 is an integral membrane protein that facilitates water transport across plasma membranes. To address the dynamics of Arabidopsis thaliana PIP2;1 at the single … brass bevel gearsWebbthe nAmCyan and the mCherry genes are separated by a P2A sequence that results in 2 separate proteins. It also includes BsmBI restriction sites flanking the nAmCyan gene … brass bib taps kitchenWebbmCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As a RFP, mCherry was derived from DsRed of Discosoma sea anemones … brass bib taps wall mountedWebbNucleus NLS-mCherry N-terminal 15 aa (nuclear localization signal) of tobacco c2 polypeptide, C-terminal GUS Grebenok et al. ... PIP2a-mCherry AtPIP2a, plasma membrane aquaporin Cutler et al. (2000) pCMU-APr; pCMB-APr Apoplast BCPsp-mCherry Medicago blue copper protein MtBCP1 signal peptide (23 aa) Pumplin and Harrison (2009); present … brass bib taps with upstandWebb15 maj 2014 · Transient co-expression of the GFP constructs with the tonoplast marker γ-Tonoplast Intrinsic Protein (TIP)-mCherry or the plasma membrane marker Plasma membrane Intrinsic Protein 2A (PIP2A)-mCherry and the pBIN-NA construct in leaf abaxial epidermal cells of Nicotiana tabacum was performed via the agroinfiltration procedure … brass bibcock